Fig 1: mPGES-1 was highly expressed in Hut78 cells and suppression of mPGES-1/PGE2 by CAY10526 inhibited cell proliferation. (A) Western blot and (B) immunofluorescence (×1,000 magnification) showed that the mPGES-1 expression in Hut78 cells was significantly higher than that in PBMC (P<0.05). (C) mPGES-1 was inhibited by CAY10526 in Hut78 cells indicated by Western blot. (D) PGE2 synthesis was inhibited by CAY10526 in Hut78 cells detected by enzyme immunoassay. (E) Cell viability assays revealed that the proliferation of Hut78 cells was inhibited by CAY10526. (A,C) Each protein band intensity was quantified by ImageJ software (mean ± SD, n=3), normalized to loading control GAPDH and compared to the levels of control (A, PBMC; C, 0 µM). *, P<0.05 versus control. PBMC, peripheral blood mono-nuclear cells; GAPDH, glyceraldehyde phosphate dehydrogenase.